SUB-PROGRAM 3.6 FODDER CROPS
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| DAV-104A | A uniform objective system for quality description of Australian fodder products | Peter Flinn | 03 5573 0900 | Agriculture
Victoria,
Pastoral and Veterinary Institute |
| KAK-2A | Assessment of the extent, nature and source of support for a R&D levy for the Australian fodder industry | Mr Colin Simpson | 02 6920 8216 | Kakadu Pastoral Company P/L |
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| PTP-14A | Australian Fodder Industry production Atlas | Mr Arthur Stubbs | 03 9844 1135 | Mr Arthur Stubbs |
| SAR-8A | Development of Disease Resistant, High Yielding Oat Cultivars with Enhanced Quality for Hay Production | Dr Pamela Zwer | 08 8303 9485 | South Australian Research & Development Institute |
| UWA-30A | Sulla and other forage species for southern Australia | Dr Mike Ewing | 08 9380 1776 | Centre for Legumes in Mediterranean Agriculture |
| CSA-3A | Development of a quantitative ELISA for screening fodder for corynetoxins | Dr Khin Than | 03 5227 5731 | Plant
Toxins Unit
CSIRO Animal Health |
| UQ-82A | Field testing microbial innoculants for hay preservation | Dr Peter Dart | 07 3365 2867 | University of Queensland |
Completed Projects
Research in Progress
| Project Title | Development of a quantitative ELISA for screening fodder for corynetoxins |
| RIRDC Project No.: | CSA-3A |
| Start Date: | 9/06/1998 |
| Finish Date: | 8/06/2000 |
| Researcher: | Dr Khin Than |
| Organisation: | Plant Toxins Unit
CSIRO Animal Health Australian Animal Health Laboratory PO Bag 24, GEELONG, VIC 3220 |
| Phone: | 03 5227 5731 |
| Fax: | 03 5227 5555 |
| Email: | khin.than@dah.csiro.au |
| Objectives |
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Current Progress |
Enzyme-link-immunosorbent-assay
(ELISA) technology developed by the CSIRO Plant Toxins Unit for measuring
trace levels of corynetoxins (CTs) and the closely related tunicamycins
(TMs) has been adapted to analyse corynetoxins in fodder. Previously available
anti-CT/TM antisera and newly generated antisera have been assessed and
two types of ELISA have been developed.
One is a conventional competitive ELISA. The other is a novel ELISA that depends on strong surface adsorption properties of the CTs. The former ELISA has been shown to specifically detect not only the known CTs but also water soluble substances, likely to be closely related to the CTs. Their toxicity is not known. The other ELISA specifically detects only the known CTs. It has proven to have greater reliability and sensitivity and is the preferred method for analysis of CTs in fodder. Application of the assays depends on effective methods for extracting CTs from fodder. This is the current focus of the project. Alcohols, such as methanol and ethanol, are effective extractants, but the volumes required for extraction raise concerns with cost and safety. An aqueous extractant is preferred, but these also extract substances that interfere with the assay. A solution to this problem is being sought. |
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Last updated: 6 September 1999
Copyright © RIRDC
http://www.rirdc.gov.au/99comp/eoi2.htm