Rural Industries
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RIRDC Completed Projects in 1999-2000 & Research in Progress as at June 2000
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COMPLETED PROJECTS |
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| UWA-30A | Sulla and other forage species for Southern Australia | Dr Mike Ewing | (08) 9380 1876 | Centre for Legumes in Mediterranean Agriculture |
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COMPLETED PROJECTS prior to 1999-2000 |
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| CSA-3A | Development of a quantitative ELISA for screening fodder for corynetoxins | Dr Khin A Than | 03 5227 5731 | CSIRO Animal Health |
| UQ-25A | Microbial inoculants for hay | Peter Dart | 07 365 2867 | University of Queensland |
| CSJ-1A | Objective specification of the quality of hays & fodder | Sue Baker, Robyn Dynes, Barrie Purser & David Henry | 08 9333 6000 | CSIRO Animal Production |
| DAV-104A | Uniform objective system for quality description of Australian fodder products | Peter Flinn | (03) 5573 0900 | Agriculture Victoria |
| DAW-28A | Development of exports of oaten hay to Japan - alternative shipping methods | CPF De Lima & R Nussey | 09 368 3494 | Department of Agriculture |
| SAR-2A | Establishment of annual ryegrass toxicity (ARGT) testing services and protocols for the Australian export hay industry | Alan McKay | (08) 8303 937 | South Australian Research & Development Institute |
| KDI-6A | A publication on fodder systems for Australian farming | M. Evans | 09 478 3343 | Kondinin Group |
| DAN-88A | Haymaker – extension technology for lucerne management | Ian Collett | 02 6763 1100 | NSW Agriculture |
R&D Management Monitoring and Review
| KAK-2A | Assessment of the extent, nature and source of support for a R&D levy for the Australian fodder industry | Mr Colin Simpson | 02 6920 8216 | Kakadu Pastoral Company P/L |
| UQ-23A | An evaluation of the fodder potential of tree legume species | R.C. Gutteridge | 07 3365 2062 | Department of Agriculture |
Plant Breeding & Germplasm Evaluation
Completed Projects prior to
1999-2000
Plant disease, weed, pest and micro-organism management
| Project Title | Development of a quantitative ELISA for screening fodder for corynetoxins |
| RIRDC Project No.: | CSA-3A |
| Researcher: | Dr Khin A Than |
| Organisation: | CSIRO Animal Health
Private Bag 24 (5 Portarlington Road) Geelong Vic 3220 |
| Phone: | 03 5227 5731 |
| Fax: | 03 5227 5555 |
| Khin.than@dah.csiro.au | |
| Objectives |
· To demonstrate an enzyme-linked-immuno-sorbent-assay (ELISA) for screening fodder for corynetoxins that will be useful in quality assurance of fodder and help to ensure that corynetoxin contaminated fodder does not cause annual ryegrass toxicity (ARGT) in sensitive markets such as Japan. |
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Background |
Hay contaminated with corynetoxins causes ARGT in livestock, a problem found only in Australia. Oaten hay from Australia was responsible for several incidents of ARGT in Japan that led to export restrictions. Current QA of hay uses an ELISA for the bacterial source of the corynetoxins rather than the toxins themselves. While this has so far been successfully in preventing further incidents of poisoning it is an indirect way of ensuring that fodder is safe. Some hay that shows a strong response when assessed by the bacterial assay may be safe for stock. It was therefore considered desirable to establish a method for detecting and measuring the corynetoxins to allow better management of the problem. CSIRO had patented technology that was considered to be suitable for the purpose but this needed to be adapted for specific application to fodder. |
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Research |
Antisera against the corynetoxins can be induced in sheep when they are injected with a modifiedform of the corynetoxins conjugated to a protein. Existing supplies of anti-corynetoxin antisera and newly generated sera where assessed for their suitability for use in a corynetoxin ELISA for application to fodder samples. The conditions for conducting the ELISA where optimised. This involved research into coating microtitre plates with toxin conjugates, selection of assay buffers and assay conditions. The potential for extractives from the fodder to interfere with the assay was also assessed. The solvent for extracting trace levels of toxin present was also selected bearing in mind cost, safety and efficiency. The ELISA was then validated against a confirmatory HPLC assay before it was applied to 110 fodder samples. |
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Outcomes |
The assay was shown to be effective for application to fodder samples. It was found to be capableof measuring corynetoxin down to 40 parts per billion. It offers a means of determining the safety of fodder for stock. The corynetoxin ELISA will now need to undergo testing in several laboratories to ensure its usefulness to the fodder industry. An officer from Agriculture WA has been trained in its use. Similar training will be offered to SARDI. Inter-laboratory testing will be conducted by CSIRO to ensure that the ELISA meets acceptable standards for QA of fodder. |
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Implications |
Following a period in which to prove its robustness and suitability for routine application to fodder samples, during which some fine tuning is anticipated, it will be available to the fodder Industry for use in assuring the safety of fodder with the potential to cause ARGT. It is expectedto enable the fodder industry to better manage the problem of ARGT and to protect the industry from future trade disruptions. The reagents and standards needed for the assay are specialised products and their future availability needs to be established if the assay proves to be importantfor the fodder industry. |
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Publications |
Than KA, Cao Y, Michalewicz A,
Cockrum PA, Olsen V and Edgar JA (1999). Novel quantitative ELISA
for corynetoxins. AFA’99: Society for Food and Agricultural Immunology’s 5th International Conference: Agri-Food Antibodies ’99; 1999 Sep 14-1999 Sep 17: Norwich, U.K. 1999 [Abstr.] |
Quality standards
| Project Title | Objective specification of the quality of hays & fodder |
| RIRDC Project No.: | CSJ-1A |
| Researcher: | Sue Baker, Robyn Dynes, Barrie Purser and David Henry |
| Organisation: | CSIRO Animal Production
Private Mail Bag 5 PO Wembley WA 6913 |
| Phone: | 08 9333 6000 |
| Fax: | 08 9387 8991 |
| s.baker@ccmar.csiro,au; r.dynes@ccmar.csiro.au; purser@ccmar.csiro.au; d.henry@ccmar.csiro.au | |
| Objectives |
· To provide the Australian hay industry (domestic and export) with simple and uniform quality standards based upon objective specification, and |
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Background |
The value of hays traded in Australia is about $600M, and of this hay exported mostly to Japan, Korea and Taiwan makes up about 11% of hay traded. Livestock producers understand that differences in hay and fodder quality should be reflected in animal performance. Despite this, prices paid for hays do not reflect likely performance of animals consuming them. The United States has been active in developing and putting in place quality standards for hays, and prices are based upon objectively-measured hay quality. Objective, performance-based quality standards that are accepted by the industry will provide a sound basis upon which price differentials can be determined. Objective, performance-based quality standards will also enhance the ability of exporters of hay to capture and retain markets in the face of increasing competition from other exporting countries, particularly the United States. The livestock industries will benefit by an assurance that performance will be related to price paid for hay or fodder |
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Research |
Hay and fodder nutritive value is sometimes confused with hay and fodder quality (also known as feeding value). Nutritive value is determined by digestibility and efficiency of utilisation of nutrients, whereas feeding value is determined by nutritive value and voluntary feed intake. Thus hays and fodders of similar nutritive value can differ substantially in feeding value (quality). Biomechanical characters of hays reflect how difficult the hay is for the animal to chew, and are important in constraining voluntary feed intake by ruminants. Hay quality characters, including biomechanical characters, were evaluated to determine which of them best described the quality of the hays in terms of their feeding value, by feeding 35 cereal hays from 3 growing seasons to sheep. |
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Outcomes |
Characters of hay that can be measured in laboratory analyses can be used as predictors of animal performance. The characters that best predict constraint to voluntary feed intake of cereal hays were shear energy (a biomechanical character), neutral-detergent fibre (NDF), and crude protein content. From a prediction of constraint to intake, voluntary feed intake can be predicted with acceptable reliability. It was confirmed that biomechanical characters can be predicted using near infra-red reflectance spectroscopy (NIRS) (patents pending). |
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Implications |
Adoption by the Australian hay and fodder industry of quality standards that reflect likely animal performance is important for the competitive of the industry and for customer confidence. Using this approach Australia is gaining an increasing market share it its export markets. |
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