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RIRDC Completed Projects in 2005-2006 & Research in Progress as at June 2006

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To Pasture Seeds Research in Progress
 
2.6 PASTURE SEEDS - COMPLETED PROJECTS
PROJECT
No
PROJECT TITLE RESEARCHER PHONE ORGANISATION

Improved Production Efficiency

CSE-91A Improving lucerne pollination with leafcutter bees – stage 2 Dr. Denis Anderson (02) 6246 4148 CSIRO Entomology

Incorporate Environmental Considerations in Sustainable Production Systems

DEB-5A Water balance and quality study for lucerne seed production resources by an underground aquifer Mr. James De Barro (08) 8756 2777 De Barro Agricultural Consulting

 
2.6 PASTURE SEEDS - COMPLETED PROJECTS prior to 2005-2006
PROJECT
No
PROJECT TITLE RESEARCHER PHONE ORGANISATION

Improved Production Efficiency

UWA-65A Seed production limits to sulla and purple clover as fodders Dr. Mike Ewing (08) 9380 1876 University of Western Australia

Export and Import Replacement Opportunities

SAR-25A Determine international standards for disease testing of lucerne seed Dr Kathy Ophel-Keller (08) 8303 9368 SARDI
SAR-10A Development of a bacterial wilt test to facilitate the export of lucerne seed Dr Kathy Ophel-Keller (08) 8303 9368 SARDI
SBS-1A Seed production of creeping vigna Dr Donald S Loch (07) 32861488 QLD Dept of Primary Industries
DAS-31A Development of export markets for cold tolerant annual medics Dr Ian Kaehne (08) 8266 8333 SARDI
SED-2A Developing management systems for European varieties of white clover for multiplication in Australia and re-export Mr Ted Higgs (08) 8234 9333 Seedco
SED-3A Development of Four Fodder Legume Seed Species for Export Dr Ross Downes (08) 8234 9333 Seedco
SED-8A Development of varieties of six species of fodder legumes for sowing seed and hay export Dr Ross Downes (08) 8234 9333 Seedco
DAW-50A Development of alternative pasture legumes for use in medium rainfall agricultural regions with untapped subsurface water, and to enhance product range in the seed and rhizobial inoculant export markets
 
 

Dr S J Carr


 
 

(08) 9368 3870


 
 

CLIMA

Availability of Technical and Economic Information

DAV-169A Practical guidelines for sub clover seed production in south eastern Australia Ms. Angela Avery (02) 6030 4500 Department of Natural Resources & Environment (Vic)
DAV-106A A model for expansion of the Australian domestic temperate pasture seed market Mr Peter Schroder (03) 5572 3825 Rural Consulting
HAS-5A A study of the costs of production of lucerne, medic and clover seeds Hassall & Associates Pty Ltd (02) 9241 5655 Hassall & Associates Pty Ltd
KDI-7A Pasture establishment and management publication Mark Casey (08) 9478 3343 Kondinin Group
DAN-75A Lucerne seed check: a management support system approach to improving lucerne seed profitability RB Walker (02) 6391 3174 NSW Agriculture
DAV-57A Development of rapid biochemical methods for the identification of medic cultivars M Mebalds & J Woodward (03) 210 9226 Agriculture Victoria
DAN-76A Developing an agronomic package to maximise profitability of sub clover seed crops Jim Virgona (02) 6938 1999 NSW Agriculture
DAW-60A On farm evaluation of the adaptation, distribution and potential value of lucerne in WA Roy Latta (08) 9821 1755 Agriculture WA
UA-24A Survey to determine demand factors in pasture legume seed usage in Australia P G Tow (08) 8303 7857 University of Adelaide

Improved Production Efficiency

DAV-196A Effective weed control for the Australian white clover seed industry Ms. Penny Riffkin (03) 5573 0900 Department of Primary Industries (Vic)
US-85A Development of an integrated control strategy for "lucerne yellows" disease in lucerne Dr. Geoff Gurr (02) 6360 5551 The University of Sydney
CSE-86A Improved pollination of seed lucerne Dr. Denis Anderson (02) 6246 4148 CSIRO Entomology
IPB-1A Evaluation of lucerne varieties for seed yield and strategies to enhance seed productivity Dr. Ross Downes (02) 6255 1461 Innovative Plant Breeders Pty Ltd
DAN-163A Development of new alternative legumes and appropriate weed control technology Mr. Graeme Sandral (02) 6938 1850 NSW Department of Agriculture
DAV-144A Improved seed production: "Towards 1.5 tonnes" Ms Angela Avery (02) 6030 4500 DNRE Victoria
DEB-1A Evaluating and managing lucerne seed wasp in lucerne seed crops Mr James De Barro (08) 8755 3133 De Barro Agricultural Consulting

Improved Production Efficiency

DAV-94A Management of phytophthora root rot in subterranean clover for a viable pasture seeds industry Dr R Aldaoud, W Guppy, Dr S Flett, T Cook (03) 5833 5222 Agriculture Victoria
US-75A Occurrence and severity of ‘lucerne yellows disease in lucerne seed crops Dr Geoff Gurr (02) 6369 5551 Orange Agricultural College
CMS-1A Strategies to successfully establish and manage a productive dryland lucerne stand M Stanley & R Christinat (08) 8755 1295 Crop Monitoring Services
UA-27A Herbicide failure and the need for integrated weed management in legume pasture seed crops Stephen Powles (08) 8303 7237 University of Adelaide
DAW-36A Developing effective rhizobial inoculants for lucerne in WA Pedro Evans (08) 9821 3343 Agriculture WA
DAS-33A Control of Rhizoctonia Burr Rot of subterranean clover Alan Dube 08) 8226 1877 SARDI
ARM-1A Review of weed control in lucerne, medic and sub clover seed crops Charles Kidd (08) 8842 3591 Australian Rural Management Services Pty Ltd 
DAS-32A Alfalfa Mosaic Virus (AMV) survey in Lucerne and Medic Seed and development of rapid tests Evita Alberts (08) 8372 2349 PIRSA

Environmental Issues for Industry

DEB-3A A water balance study for lucerne seed production resourced by an underground aquifier Mr. James de Barro (08) 8756 2777 De Barro Agricultural Consulting
HS-1A The development of a more effective harvesting method for shaw creeping Vigna (Vigna parkeri) Mr Bob Campbell (07) 5523 4543 Heritage Seeds Pty Ltd
DAQ-148A Improved handling of chaffy grass seeds Dr Donald S Loch (07) 3286 1488 QLD Dept of Primary Industries

Support Industry Quality Assurance and Self Regulation

SAR-48A Confirmation of pest free area status from bacterial wilt of lucerne in export seed production areas Mr. Rob Rees (08) 8226 0446 South Australian Research and Development Institute
SED-6A Alternative seed certification schemes in Europe and USA for adoption in Australia Max Jongebloed (08) 8231 6675 Seedco

Completed Projects - Improved Production Efficiency

Project Title Improving lucerne pollination with leafcutter bees - stage 2
RIRDC Project No.: CSE-91A
Researcher:  Dr Denis Anderson
Organisation: CSIRO Entomology
Phone: (02) 6246 4148
Fax: (02) 6246 4000
Email: Denis.Anderson@csiro.au
Objectives The outcome will be increased profitability for the lucerne seed industry, achieved by improving seed yield through the use of leafcutter bees as pollinators. The deliverables: · Cost-effective quarantine procedures for importing Canadian leafcutter bees.

· Improved methods for managing imported Canadian leafcutter bees under Australian conditions.

Background Seed yields from lucerne crops in Australia are, on average, much smaller than those obtained in Canada and the United States. The higher overseas yields are mostly attributed to better pollination achieved through the use of the leafcutter bee, Megachile rotundata. The establishment of this bee in Australia, and its wide adoption as a pollinator of lucerne, will improve lucerne seed yields.

Major obstacles have prevented the rapid establishment of the leafcutter bee in Australia. During an earlier stage of the current project the complex quarantine and environmental issues involved with importing the bee from Canada and getting it established in the field were resolved. The thrust of the current project was to assist the rapid establishment of the bee in Australia by improving importation and quarantine processes to enable very large numbers of bees to be imported in single shipments.

Research Approximately 1 million leafcutter bees were imported from Canada during each year of the project (2002/03, 2003/04 and 2004/05). Shipping procedures were streamlined and shorted, reducing bee losses. Upon arrival in Australia the bees were introduced into a high security quarantine centre at CSIRO Entomology in Canberra. Modifications made to the way bees were processed in quarantine, particularly modifications made to containers used to remove bees from incubation chambers, resulted in faster processing times and the release of healthier bees. Improvements made to on-farm practices for managing bees released from quarantine, particularly to the ways bees were released and held on crops, resulted in fewer bee losses.

Recovery of cells (leaf-covered cocoons containing immature new bees) from each batch of released bees was below expectation, being adversely affected by poor weather shortly after the bees were released, or by inappropriate insecticide use once the bees were established on crops. The holding of recovered cells at 2oC for two days during the over-wintering phase was found to be an effective control against a local parasitoid, Melittobia hawaiensis.


 
Outcomes · Streamlined processes for safely importing large numbers of Canadian leafcutter bees in single shipments.

· Improved methods for (a) delivering imported bees from quarantine to lucerne crops and, (b) managing leafcutter bees on lucerne crops under Australian conditions.

· A DNA based diagnostic method for distinguishing imported leafcutter bees (Megachile rotundata) from native leafcutter bee species.

Implications This project has allowed for large numbers of leafcutter bees to be imported from Canada in single shipments, thus overcoming the last major obstacle for establishing leafcutter bees in Australia. It is now up to the Australian lucerne industry to exploit this opportunity and move toward establishing the bee as a means to improving lucerne seed yields.
Publications Lawrence, L. & Anderson, D. (2004). Leafcutter bees lift lucerne seed production. Farming Ahead, 152: 60-62.

Lawrence, L. & Anderson, D. (2004). Using leafcutter bees to improve the pollination of seed lucerne. Australian Grain, Nov-Dec, i-ii.


 

Completed Projects - Incorporate Environmental Considerations in Sustainable Production Systems

Project Title Water balance and quality study for lucerne seed production resources by an underground aquifer
RIRDC Project No.: DEB-5A
Researcher:  Mr James De Barro
Organisation: De Barro Agricultural Consulting
Phone: (08) 8756 2777
Fax: (08) 8756 2778
Email: dbac@lm.net.au
Objectives · Quantify the movement of salt from the applied irrigation water, through the soil, beyond the lucerne root zone en route back to the ground water during and between irrigation seasons.

· Produce a reliable, cost-effective method for monitoring salt accessions in irrigated lucerne seed production. Combined with the water balance study (DEB-3A) and undertaken in the same trial sites, the research will provide information to assist the understanding and management of irrigation water quality both in the research area as well as irrigated lucerne elsewhere. The research is important in developing water quality parameters for use in water allocation policy and licencing systems and is critical for the long-term future of irrigated lucerne seed production in the southeast region of South Australia. The research is pivotal in maximising the irrigation volumes available to lucerne seed producers and forces the DWLBC to be fair and honest in its development of the future licencing system.

Background The irrigated lucerne seed producer’s ability to irrigate provides an opportunity to obtain yields above that of a dryland system and generate an income that otherwise would not be possible. The right to irrigate comes with inherited responsibilities to use the resource in an efficient and ecologically sustainable manner. Such responsibility is under increasing political and social scrutiny as the demand for the resource increases. Present day and future lucerne seed irrigators need to be seen by all sections of the community to be actively researching a solution to efficient and effective water use thereby justifying existing demand for irrigation in a sustainable system. 

The research aimed to develop a reliable method for quantifying the movement of salts from the applied irrigation water, through the soil, beyond the root zone en route back to the ground water during and between irrigation seasons. Research such as this is needed for irrigated lucerne seed producers to quantify the impact of their irrigation practices on their water quality. This information will provide the benchmark data for development of sustainable irrigation practices for the future. In the short and long term the research is essential for irrigated seed producers to quantify the direct impact of their irrigation practices on the underground aquifer to enable them to support their requirements in water allocation policy and licencing system implementation. 

This research is significantly funded from irrigated lucerne seed producers and will provide information upon which to develop improvements in efficiency whilst at least maintaining production and will be evidence to relevant government authorities and communities of irrigators consciousness of the importance of developing management systems that permit profitable yet ecologically sustainable irrigation water use. Irrigators require their own independent research to collect data that can be used to support their business interests and they need to be an active participant in the research so as to achieve a fair and equitable result.

Research Five field trial sites were established in lucerne seed crops in the Keith region of South Australia. Four of the trial sites were situated on soil types typical of border check flood irrigation in the Tatiara Prescribed Wells area. A fifth site was established under a centre pivot and was located in the Coombe area, north of Keith in Tintinara Coonalpyn Prescribed Wells Area. The centre pivot site was only installed to monitor the ground water salinity and lysimeter data.

The water balance aspect of this project was performed for the flood irrigation according to the methodology outlined in ‘Dividing the Droplet’ (RIRDC Pub.No. 05/116). 

Three lysimeters were created at each site and proved to be an effective means of sampling soil water. Lysimeter depths were approximately 1, 2 and 3 metres deep. The lysimeter enabled soil stored water to be sampled via the ceramic tip at the depth of interest. The soil water sample was analysed for salinity using the DWLBC salinity tester at the Naracoorte office. This is the same device used for salinity analysis of the district’s observation wells.

Each flood site had a groundwater monitoring well installed. The wells were situated in the middle of the bay of the flood irrigated crops and halfway between the pivot’s centre and circumference. The wells were constructed to the same depth as the irrigation bore supplying the irrigation water. Each well had a continuously recording water level and salinity sensor installed. The well was located adjacent to the C-Probe and lysimeters. The water level and salinity sensor was calibrated by manual sampling. The data was recorded and transmitted by telemetry to a base computer.

Completed Projects (prior to 2005-2006) - Improved Production Efficiency

Project Title Solving seed production limits for sulla and purple clover as fodders
RIRDC Project No: UWA-65A
Researcher:  Dr Mike Ewing
Organisation: University of Western Australia
Phone: (08) 9380 1876
Fax: (08) 9380 1140
Email:  mewing@cyllene.uwa.edu.au
Objectives The project will deliver for sulla and purple clover: · Commercial cultivars of sulla and purple clover with good adaptation and acceptable harvest/processing characteristics.

· A detailed understanding of current best practice for commercial harvest and seed processing of the target species.

· A detailed understanding of the plant characters that will confer improved harvestability and handling.

· Selection methodologies for use in practical plant breeding programs for enhanced harvest and handling characteristics.

· Cohorts for further field evaluation that will provide second generation cultivars with elite harvest/processing abilities.

Background Purple clover (Trifolium purpureum) and sulla (Hedysarum coronarium) were identified in RIRDC project UWA-30A "Sulla and Other Forage Species for Southern Australia" (Publication No. 00/41) as being highly productive species with great potential as high quality forage legumes in new farming systems for southern Australia. A major constraint to their adoption, however, is in the provision of low-cost seed, while Paratta, the original purple clover cultivar, failed commercially due to its susceptibility to clover scorch disease (Kabatiella caulivora). 
Research  The project identified 74 accessions of purple clover with resistance to clover scorch disease. This is the first record of resistance to the disease. The 13 promising purple clover genotypes in UWA-30 were further evaluated in WA and NSW. CPI 139465 and CIZ12Pur-A, both with resistance to clover scorch and good threshing characteristics, were identified as the best performing varieties and underwent selection for uniformity of leaf markers. CPI 139465NM, has subsequently been short-listed for cultivar release. Eighteen elite lines have also been selected for national field evaluation trials.

Field evaluation of promising sulla genotypes from UWA-30 identified HRN83-A as one of the best performing line. It has been short-listed for cultivar release. New softseeded sulla genotypes that would not require post harvest dehulling have also been identified. Their further development is the subject of a new RIRDC project. 

Threshability experiments, using both commercial and experimental machinery, were conducted on both species to develop improved seed processing efficiencies.


 
Outcomes  CPI 139465NM purple clover and HRN83-A sulla, have been short-listed for release as new cultivars, subject to satisfactory pre-basic seed increase. A final decision on their release will be made in early 2006, in consultation with RIRDC.

Elite germplasm of both species, with improved seed processing capabilities and disease resistance, has also been identified for further variety development. 

Reliable screening techniques in both species have been developed for threshability, while the mechanical, physical and climatic parameters for harvesting are better understood. 

Implications  This project has taken two relatively unknown species to Australian agriculture to the point of commercial release. This will provide industry with two new highly productive and sustainable forage options. While some seed production and processing hurdles still need overcoming to markedly reduce seed costs, this project has identified genotypes and harvesting technologies to make significant inroads towards this objective.
Publications Gajda, K. and Snowball, R. (2002). Morphological diversity of purple clover. Proceedings of the 12th Australasian Plant Breeding Conference, September 2002, Perth Western Australia, 218-221.

Completed Projects (prior to 2005-2006) – Export and Import Replacement Opportunities

Project Title Determine international standards for disease testing of lucerne seed
RIRDC Project No: SAR-25A
Researcher:  Dr. Kathy Ophel Keller
Organisation: South Australian Research and Development Institute (SARDI)
Field Crops Pathology Unit
Phone: (08) 8303 9368
Fax: (08) 8303 9393
Objectives · Development of accredited protocols for bacterial wilt pathogen of lucerne seed.
Background The export trade in lucerne seed is worth $12 million pa in Australia. All importing countries require declarations of freedom from bacterial wilt. All importing countries use tests of varying standards to accept or reject shipments. The lack of international standards for bacterial wilt (Clavibacter michiganensis subsp. insidiosus) or Verticillium wilt of lucerne exposes seed companies to potentially large losses if a shipment is rejected. 

The International Seed Health Initiative (ISHI) was formed by the Seed Trade Federation (FIS) to standardise disease testing and remove restrictions based on non-acceptance of disease test standards between countries. An ISHI Technical Committee was formed in 1998 to standardise testing for herbage and grass seeds. Lucerne is the first seed crop to be evaluated. There are two objectives for lucerne: to establish standards for Bacterial Wilt and Verticillium Wilt, and to select the test most suitable for routine use by testing laboratories.

Research  In 1999/2000 the Technical Committee evaluated three selective media for bacterial wilt and the ELISA test developed by SARDI (SAR10A). The group also examined variation between Cmi strains internationally and commenced a comparison of methods for Verticillium wilt testing. SARDI participated in evaluation of all protocols and coordinated the evaluation of the ELISA test. 
Outcomes  The SARDI ELISA test worked well in 3 out of 5 laboratories. It was evident from the ELISA and media kit ring testing that stability of reagents and cultures shipped overseas is an issue. There will be continuing work to stabilise reagents to finalise adoption of the ELISA test for bacterial wilt.
Implications  Acceptance of the test by the International Seed Testing Association will facilitate lucerne seed export.
Publications J Gooden, DA Samac, D Caffier, K Ophel Keller and J. Sheppard (2000). Method validation by ringtesting to establish international standards for seed testing: a case study. Poster presentation. International Conference on Plant Pathogenic Bacteria (PPB2000).

Completed Projects (prior to 2005-2006) – Export and Import Replacement Opportunities

Project Title Development of a bacterial wilt test to facilitate the export of lucerne seed
RIRDC Project No: SAR-10A
Researcher:  Dr. Kathy Ophel Keller
Organisation: Field Crops Pathology Unit, South Australian Research and Development Institute (SARDI)
Phone: (08) 8303 9368
Fax: (08) 8303 9393
Objectives · A specific and rapid detection assay for bacterial wilt of lucerne will be developed.
Background The lucerne seed industry has a total value of $8 to $10 million pa. All countries importing lucerne seed require declarations of freedom from bacterial wilt. Bacterial wilt of lucerne is caused by Clavibacter michiganense subsp. insidiosus (Cmi). Existing methods of detecting the pathogen are unreliable because they are based on plating of bacteria and subsequent visual examination of bacterial colonies. The bacterium is very difficult to isolate on growth media. A test was required which would be more sensitive and reliable and did not require plating.
Research  The research project has developed an ELISA-based detection for Clavibacter michiganense subsp. insidiosus, (Cmi), the causal agent of Bacterial Wilt in lucerne, in seed.
Outcomes  The availability of a test for bacterial wilt will facilitate export of lucerne seed. The test developed in this project will be tested against other methods of bacterial wilt detection as part of an International Seed Health Initiative aimed at standardisation of seed testing internationally.
Implications  Availability of the test and acceptance of the test by the International Seed Testing Association will facilitate lucerne seed export.
Publications International Seed Testing Association/ International Seed Health Initiative Comparative Test Plan: Bacterial Wilt of Lucerne. Published on ISTA Web Site.

Completed Projects (prior to 2005-2006) – Export and Import Replacement Opportunities

Project Title Seed production of creeping vigna
RIRDC Project No: SBS-1A
Researcher:  Dr Donald S. Loch
Organisation: Queensland Department of Primary Industries
Phone: (07) 3286 1488
Fax: (07) 3286 3094
Email lochd@dpi.qld.gov.au
Objectives In relation to Vigna parkeri,
· to document development and seed production of seed crops;

· to determine environmental factors controlling flowering, and to explore possible manipulation of flowering in seed crops;

· to develop appropriate commercial seed production strategies and methods, including defoliation and irrigation practices and herbicide use;

· to enhance field establishment by developing effective methods for reducing hardseededness; and

· to evaluate seed production characteristics of Vigna germplasm approaching release.

Background Vigna parkeri (creeping vigna), a cool-growing highland tropical legume from East Africa, is well adapted to the humid Australian subtropics (>1100 mm rainfall annually). Its growth habit - combining a stoloniferous mat with twining lateral ‘vines’ - ensures that it is very persistent under heavy grazing, and allows it to form stable and productive mixed pastures with a range of grasses including strongly competitive species such as kikuyu, setaria and signal grass. The cv. Shaw (released in 1984) is recognised as the premier summer legume for dairy pastures in coastal southern Queensland and northern NSW, but its widespread commercial use is constrained by seed availability.
Research  The pattern of vegetative and reproductive development, and the progressive production and loss of seed in a commercial crop of cv. Shaw were recorded. The effects of day-length and moisture stress on flowering were investigated in controlled environment experiments. Pre- and post-emergence herbicides (for selective control of broadleaf and grass weeds) were screened in glasshouse and field experiments. The effectiveness and reliability of different methods of reducing hard seed levels were assessed. Seed production characteristics of promising new Vigna accessions were assessed under nursery conditions
Outcomes  ‘Shaw’ did not respond to short-day conditions under controlled environment conditions, and both high temperatures and moisture stress were detrimental to plant growth. The safest herbicides for post-emergence control of broadleaf weeds are the imidizolinones (imazethapyr, imazaquin), bentazone, acifluorfen and flumetsulam, but there is the risk of some stunting of V. parkeri seedlings. The strategic use of selective grass herbicides at sub-lethal rates in late summer/early autumn was investigated to manipulate the level of the grass component in mixed swards for maximum seed production potential. In wet years, Colletotrichum and Rhizoctonia spp. can devastate maturing seed crops. Hot water treatment (20 minutes at 70°C) was the most effective means of reducing hard seed levels.

 
Implications  V. parkeri is best grown for seed mixed with a grass (eg. Pennisetum clandestinum), which can be slowed down by a sub-lethal dose of herbicide (haloxyfop, sethoxydim, etc) in January to provide a strong leafy canopy for flowering. It should not be cut back during the heat of summer, and requires good soil moisture throughout the growing season to develop a strong vegetative framework prior to flowering in February-March. Flowering in cv. Shaw appears to be triggered by a significant rainfall event once the temperature falls consistently below a temperature threshold, though the field behaviour of a second blue-flowered line (CPI 100846) is suggestive of a short-day response.
Publications Wade, J. (1999) Optimising the conditions for growth and reproductive development of Vigna parkeri, a sub-tropical legume. B.Sc. Honours Thesis, Department of Botany, University of Queensland. 30 pp.

 

Completed Projects (prior to 2005-2006) – Export and Import Replacement Opportunities

Project Title Development of export markets for cold-tolerant annual medics
RIRDC Project No: DAS-31A
Researcher:  Dr Ian Kaehne
Organisation: Northfield Research Laboratories
Phone: (08) 8266 8333
Fax: (08) 8261 4688
Objectives To develop export markets for cold-tolerant annual medics by:
· further demonstrating that cold-tolerant cultivars with export potential can be economically grown in Australia

· consolidating and servicing an established overseas network of collaborators who are testing the adaptation of cold-tolerant medics in target countries

· supporting product development and encouraging evaluation of farming systems for using medics in target countries

· developing those procedures which will achieve further product improvement.

Background Annual medics were developed in southern Australia for use in pastures and in ley farming systems in conjunction with cereal crops. Since the 1970s, this practice has also been advocated in the Mediterranean and Western Asian regions of the Old World and, as a result, significant quantities of Australian medic seed has been exported to these regions. Most Australian medic cultivars have been developed from naturalised introductions or by screening accessions from overseas, and all have been developed in the Mediterranean type environment of southern Australia. In export destinations, except for certain areas, the lowest extremes of winter temperatures are generally lower than in southern Australia. In these areas having colder winters Australian cultivars often failed because they were killed or severely damaged by frost. Consequently, in the early 1980s the export of Australia cultivators contracted as customers found that they were poorly adapted to frost-prone areas. The redevelopment of the export industry therefore required the identification of genotypes of annual medics which were adapted to the frost regimes which would be expected in target areas, and which also would have seed yielding capability so that they could be produced economically for export.
Research  In Australia sites were established at Mallala and Yongala in SA, to determine levels of seed production and the grazing tolerance of the various cultivators and lines selected for evaluation. Mallala is a centre for medic seed production, and Yongala is a potential centre but is sometimes subject to severe frosts.

From 1991 to 1993 various lines and check cultivators were also evaluated at La Fage in southern France, and at Tulake in northern California, USA. Attempts to evaluate material in north Africa were frustrated by drought (morocco), and civil unrest (muslim fundamentalism in Algeria).


 
Outcomes  As a result of testing both in Australia and overseas, a short list of thirteen genotypes from five species of Medicago have been chosen for further commercial assessment by the commercial partner, Seedco. Three of the species have not previously been advanced for commercial consideration in Australia.
Implications  There is strong commercial interest in this project from around the world. It is difficult to predict what the demand for seed will be, but a realistic estimate is 1000 tonnes per annum for markets not currently supplied with Australian cultivators.

One of the strong features of this project has been the close involvement of the commercial partner, Seedco who bring considerable experience in developing export markets.

Completed Projects (prior to 2005-2006) – Export and Import Replacement Opportunities

Project Title Developing management systems for European varieties of white clover for multiplication in Australia and re-export
RIRDC Project No: SED-2A
Researcher:  Mr Ted Higgs
Organisation: SEEDCO
Phone: (08) 8234 9333
Fax: (08) 8234 6133
Objectives Multiply European vanities of white clover for export by:
· identifying the optimum varieties suitable for multiplication in Australia

· developing optimal management systems for these species.

Background White clover seed production in Australia increased following the registration of the cultivar Haifa in March 1971, and has been concentrated in the south east district of South Australia and adjacent Western Victoria. Haifa has been the dominant cultivar grown for seed. Attempts to cultivate other species have not been very successful.
Research  An overview of the industry was prepared and three experiments were conducted.

Experiment one compared Haifa and Huia species, also Aran, Karina, Zaoican and Grasslands Kopu were considered. Experiment two considered the species Tamar, Lune de Mai, and Crau. Experiment three gave specific regard to vernalisation and its impact on inflorescences.

Outcomes  Haifa outperformed all other species. The lower success rate of these other species is proportional to the degree that the species vary from Haifa particularly in their adaptation to high latitudes and low winter temperatures.

 

Completed Projects (prior to 2005-2006) – Export and Import Replacement Opportunities

Project Title Development of Four Fodder Legume Seed Species for Export
RIRDC Project No: SED-3A
Researcher:  Dr Ross W Downes
Organisation: SEEDCO
Phone: (08) 8234 9333
Fax: (08) 8234 6133
Objectives · To develop suitable varieties of fodder legumes so that seed can be produced in Australia for export as sowing-seed.
Background In this program, varieties of species important in Europe have been modified so that high yields of quality seed can be produced by Australian seed-growers with minimal use of chemical sprays. The disease-resistant varieties that have been developed can also be used by Australian farmers interested in exporting quality hay to Asian markets.
Research  The four species of fodder crops are: Trifolium alexandrinum (berseem clover), T. resupinatum (Persian clover), Vicia villosa (woolly-pod vetch) and V .sativa (common vetch). The research approach varied with species, but involved hybridisation, various forms of selection and progeny testing, and evaluation. Much of the research was conducted on commercial seed farms to ensure that the varieties produced are well adapted to the seed-growing environment.
Outcomes  For Persian clover, two varieties, ‘Laser’ and ‘Leeton’, from this program are in commercial production. They have high yields of seed and are rust-resistant so that fungicides are not required. For the other three species, seeds have been sown for increase so that varieties will be released for commercial use in 1995 and 1996.

Completed Projects (prior to 2005-2006) – Export and Import Replacement Opportunities

Project Title Development of varieties of six species of fodder legumes for sowing-seed and hay export
RIRDC Project No: SED-8A
Researcher:  Dr Ross W Downes
Organisation: Seedco
Phone: (08) 82349333
Fax: (08) 82346133
Objectives · To modify selected fodder species in order to enhance seed yield and forage production for improved export potential.
Background Tall-growing annual fodder legumes have a long history of use in Europe for green manure, silage and hay. In Australia they have found little acceptance in contrast to the prostrate annual legumes such as sub clover and medics used for grazing. When opportunities to export quality sowing-seed to the EU arose, Australia could provide quality through seed certification schemes but not the high seed yielding varieties of fodder legumes. This program was designed to produce the required varieties for seed export with emphasis on seed yield and disease resistance under Australian farm conditions to make the varieties competitive and requiring minimal chemical inputs to seed production. At the same time, changes in the prosperity of Australian farming systems have suggested a place for fodder legumes for hay production and in crop rotations on Australian farms.
Research  Following market research, six species were selected for modification. The aim was to increase their seed production in the southern Australian winter rainfall environment by adapting the crops’ growth patterns to seasonal rain expectation and to increase disease resistance under these conditions. Accessions were accumulated from various sources, direct selections were made in the field, and the most appropriate stocks were identified. Recombination was achieved by manually crossing plants to produce hybrids, or by allowing recombination to be achieved by cross-pollination by bees. Plants were selected in various locations and potential varieties were compared in plots. Plants were checked for disease resistance in the field or with artificial inoculation in glasshouses. Varieties commercialised were sown in nurseries for description and inspection in order to obtain Plant Breeders Rights.
Outcomes  The following outcomes were achieved:
· In common vetch (Vicia sativa) the varieties Vedura, Velero and 
Vestar were released and requirements for Plant Breeders Rights have been completed. These varieties have high seed yield potential and differ in maturity, providing the opportunity for vetch to be grown in different rainfall zones. Two other selections, one early and one late flowering are also available for commercialisation

· Two varieties of woolly-pod vetch (Vicia villosa) have also been produced. One is Capello with soft seed, while Haymaker Plus has uniform flowering and high seed yield. Both have been described for Plant Breeders Rights protection

· Elite II, a variety of berseem clover (Trifolium alexandrinum) selected for high seed yield and tolerance to Kabatiella attack has been released and PBR applied for

· In persian clover (Trifolium resupinatum var majus) the varieties Laser and Leeton were selected for rust resistance and high seed yield have been described for PBR and are available commercially

· A selection of arrowleaf clover (Trifolium vesiculosum) is performing very well in both fodder and seed production in trials and is being considered for commercialisation. 

· Despite intensive efforts to locate suitable types of red clover (Trifolium pratense) for seed production in a winter rainfall environment, all accessions evaluated flowered over an extended period and well into the summer. It appears that this crop not is suited for seed production in regions with winter rainfall.

Implications  The eight varieties developed, and three potential varieties of fodder legumes emerging from this program have significant potential for sowing-seed export. In addition, many of the varieties have considerable possibilities in Australia. They are being evaluated for weed control, for hay and silage production, as green manure crops in the cotton and rice industries, as cover crops for lucerne and as alternatives to grain legumes. They can increase the flexibility of farming rotations, fixing nitrogen and breaking disease cycles.

Completed Projects (prior to 2005-2006) – Export and Import Replacement Opportunities

Project Title Development of alternative pasture legumes for use in medium rainfall agricultural regions with untapped subsurface water, and to enhance product range in the seed and rhizobial inoculant export markets
RIRDC Project No: DAW-50A
Researcher:  Dr. S.J. Carr
Organisation: Agriculture WA/CLIMA
Phone: (08) 9368 3870
Fax: (08) 9367 2625
Objectives · To develop new deep-rooted herbaceous legume species and their root nodule bacteria adapted to the sandy and duplex soils common in medium rainfall regions of southern Australia. These species will exploit excess subsurface moisture and thereby reduce waterlogging and secondary salinity. They will increase on-farm productivity by extending the effective growing season, and their development will be exploited by the seed and inoculant export industries to expand product range in Europe and North Africa.
Background The underlying concept was to identify species able to remain green for extended periods during late spring/early summer when rainfall in Mediterranean climates declines. Our assumption is that such pasture plants are able to use a higher proportion of the incident rainfall, an important consideration in decreasing the rate of secondary salinisation. Another selection criteria pertained to seed production. Historically pasture species such as sub-clover have been suction harvested in a slow, environmentally damaging and certainly non-sustainable process. We aimed to select species with high seed yielding capacity and that does not require suction harvesting.
Research  The research conducted was split between standard field evaluation of known pasture plants (eg. crimson clover) used in Mediterranean regions, and more basic research to identify new species with the attributes we considered desirable (eg. Biserrula pelecinus). In meeting the selection criteria we had set, we concluded the plant ideotypes we were targeting were generally more suited to ‘cut and carry’ types rather than regenerating farming rotational pasture species. Hence, we were not constrained by attempting to fit in with parameters such as conventional hard seed levels.
Outcomes  Three new pasture legumes (Trifolium vesiculosum cv Cefalu; Trifolium incarnatum cv Caprera and Biserrula pelecinus cv Casbah) and a specific rhizobial inoculant for each cultivar were commercialised during the final stages of this research program, and the commencement of a related fodder project (UWA-30). Several other species with commercial prospects were also identified, and are subject to further evaluation.
Implications  The transition of these three new pasture legumes into commerce has recently commenced. Unlike new varieties of standard pasture species like subterranean clover which blend immediately into current management systems, these new products require additional support. The ultimate success in increasing the profitability and sustainability of agriculture in Mediterranean regions will rely heavily on appropriate developmental support from government, seed industry representatives and private agronomists.

Completed Projects (prior to 2005-2006) - Availability of Technical and Economic Information

Project Title Practical guidelines for sub clover seed production in south eastern Australia
RIRDC Project No: DAV-169A
Researcher:  Angela Avery
Organisation: Agriculture Victoria Rutherglen
Phone: 02 60 304 500
Fax: 02 60 304 600
Email:  angela.avery@nre.vic.gov.au
Objectives ·1 To increase the profitability, sustainability and the market profile of the subclover seed production industry (including the RIRDC Pasture Seed Program) through the development and publication of production based guidelines.
Background Trifolium subterraneum (subclover) is an important legume for farming systems in South Eastern Australia. The subclover seed industry is small when compared to other agricultural industries but is of great importance to Australian agriculture as it supplies affordable, high quality legume seed to many grazing and cropping enterprises. Economic viability and long-term sustainability are major challenges for this industry, with industry analysis revealing increased production costs, declining seed yield and a fall in the number of growers. The continued ability of Australia to produce quality seed at an acceptable price for both the domestic and export markets is critical. Guidelines and benchmarks to support decisions relating to subclover seed production will increase the competitiveness and economic and environmental sustainability of the industry. Before this project there were no guidelines available for the production of subclover for seed production.
Research  Guidelines based upon information from past and current research, seed certification officers and case studies of leading farmers/enterprises have been developed. Scientists, farmers and industry for accuracy, relevance and practicality reviewed these guidelines before being published. The guidelines detail critical areas for the production of quality seed, for example soil phosphorus levels, weed densities and disease and grazing sequences to optimise seed production. 
Outcomes/

Implications 

The guidelines provide useful information, however in some areas available data and knowledge restricted the detail and further refinement of guidelines. Clearly continued research and development is required to continue to improve efficiency and sustainability of subclover seed production. There is a need to work with subclover seed grower groups on-farm and in-crop to establish benchmarks and useful monitoring programs. While the publication is targeted to subclover growers much of the information will be relevant to all growers of subclover pastures. Improved contribution of subclover to the grazing and cropping industry is likely to build further confidence in this species.
Publications The final product of this project was the publication "Practical guidelines for subclover seed production in south-eastern Australia", RIRDC publication No R01/159

 

Completed Projects (prior to 2005-2006) - Availability of Technical and Economic Information

Project Title A model for expansion of the Australian domestic temperate pasture seed market
RIRDC Project No: DAV-106A
Researcher:  Mr Peter Schroder
Organisation: Rural Consulting
Phone: (03) 5572 3825
Fax: (03) 5572 3828
Email:  pandjschroder@bigpond.com
Objectives To develop a promotional model that will double the demand for pasture seed in temperature Australia where grazing is the main enterprise.
Background Australia produces about 28,000 tonnes of pasture seed annually with an estimated value of $67 million. Certified temperate pasture seed production statistics from 1980 – 81 to 1992 – 93 show a stagnant market situation. A National Seed Industry Workshop held in Canberra in 1990, which was funded by RIRDC, identified improving market penetration as one of six priority areas. The future viability of the pasture seed industry depends on action being taken on this. ‘Growing’ the domestic pasture seed market is particularly attractive as the infrastructure is already in place. This means expansion can be achieved quickly, at a relatively low cost and with minimal risk. The goal will not be achieved by tinkering around with product, price, promotion and place.
Research  The project involved five integrated steps:

1. Qualitative market research, from which flowed a model.

2. Implementation of the model over 2 years.

3. Evaluation of the impact of the implementation.

4. Quantify the size and attitudinal profile of the different market segments identified.

5. Testing, in other regions, to verify the validity of the model.

The first 4 steps were done in one district in south west Victoria. The fifth step involved work in several other ares in Victoria and with one rural merchandise group.

Outcomes  The strongest impression gained from the qualitative research was graziers lacked a detailed understanding of the system they managed and the key drivers of its profitability. Two main groups were identified – intensive and extensive graziers. The intensive group had a productivity orientation, aimed to run their enterprise as a business and were comfortable working with anyone who may be able to help them. The extensive group focussed on reducing costs, over coming problems and relied heavily on rural merchandisers for information. Peers influenced decisions in both groups but this was particularly strong in the extensive group.

Over 2 years 5 groups of intensive graziers were taken through a program by the author that challenged them to take a managing directors view of their business and to identify it’s key profit drivers. In this time 3 groups of intensive graziers were taken through a program, in their paddocks, by a rural merchandiser, to solve a problem which was of concern to them.

Evaluation of the program found there had been a significant change in the way pastures were considered by both groups. These changes flowed through into increased sales of pasture related inputs. Testing parts of the model in other areas indicated it could be applied successfully with very little modification. Discussions with rural merchandisers added further to this belief.

The quantitative research in the main study are found 35% of graziers fitted into the intensive group, 21% into the extensive group and would be receptive to the program and 46% were unlikely to ever become more interested in pastures no matter what approach was taken.

Implications  Change to the consumption of pasture seed will be largely determined by the actions taken by local service providers to the grazing industry. It is recommended that an extension program be put in place to sell the vision flowing from this project to these providers and ensure they have the knowledge, skills and follow up support required to get local programs well established. The follow up support is crucial because of the sharp change in mind set required by all these providers.
Publications Schroder, P M, (2001). A model for expansion of the Australian domestic temperate pasture seed market. RIRDC Publication No: R01/132.

Schroder, P M, O’Keeffe, M J and Marks, N L (1997). A model to double the demand for temperate pasture seed in Australia. Wool Technology and Sheep Breeding, 45 (1) pp 81–87.

Schroder, P M and O’Keeffe, M J. (1997). Getting more people to sow more pasture more often. 2nd Australasia and Pacific Extension Conference, Albury November 1997. Pp 541-546.


 

Completed Projects (prior to 2005-2006) - Availability of Technical and Economic Information

Project Title A study of the costs of production of lucerne, medic and clover seeds
RIRDC Project No: HAS-5A
Researcher:  Hassall & Associates Pty Ltd
Organisation: Hassall & Associates Pty Ltd
Phone: (02) 9241 5655
Fax: (02) 9241 5684
Objectives · To provide an overview of the industry, analyse the costs of production, identify the role of environmental regulation and identify future research priorities for the industry.
Background The production of temperate pasture seeds in Australia is diverse. In addition, the pasture seed industry within Australia has also been subject to significant changes in recent years. Detailed information is not available on the costs of production, assembly and distribution. This information is essential for providing some benchmarks to allow individual growers to gauge their performance and also to guide future directions for research and development (R&D). 
Research  This report was developed from available literature, the views of industry representatives and the results of a survey of forty pasture seed producers across Australia. Costs of production were analysed by species, State and by production method (irrigation vs dryland).
Outcomes  a) Profile: The annual value of production for temperate legume pasture seeds in 1999 was estimated to be $28 million. Production is highest in SA, then Victoria, NSW and WA. There are about 640 certified producers in these four States.

b) Annual costs of production: ranged from $386/ha (medics) to $661/ha (lucerne). Costs were lowest in WA. Key cost components were irrigation (where relevant), growing and harvesting costs. 

c) Environmental Regulation: none specific to pasture seed producers. Priority environmental issues included: minimising soil damage from harvesting, developing integrated management models, minimising inputs of pesticides and improving water use efficiencies. 

d) Priorities for future research: improving efficiency and effectiveness of chemicals, improving harvest technology, decreasing seed testing and certification costs, and researching new marketing opportunities for products.

Implications  This project provides a basis for future R&D as well as providing data that can be used by producers in benchmarking their costs of production.
Publications Hassall & Associates (1999) A study of the costs of production of lucerne, medic and clover seed in Australia, RIRDC Publication No R01/022.

 

Completed Projects (prior to 2005-2006) - Availability of Technical and Economic Information

Project Title Pasture establishment and management publication
RIRDC Project No: KDI-7A
Researcher:  Mark Casey
Organisation: Kondinin Group
Phone: (08) 9478 3343
Fax: (08) 9478 3353
Objectives To survey farmers on their current pasture practises
· To collate information on innovative methods of pasture establishment, renovation and management

· To publish a 300-400 page manual on the topic

Background Pastures are an integral part of Australian farming, and considerable research in directed at solving various pasture problems. New establishment and management strategies are being adopted by farmers, but much of the information resulting from pasture research does not effectively reach the farmer.

Surveys have shown that younger farmers particularly, do not have the knowledge on pasture issues that their fathers had. This has lead to a general failure to maintain soil fertility levels under pastures, and to inadequate control of pests and diseases; a failure to replace pastures which have degenerated; and, a failure to adopt sustainable grazing practices.

The publication resulting material (case studies on successful practices etc) was produced as a pasture manual.

Research  Following surveys of farmer practices in relation to pasture establishment and management, topics were identified on which contributions could be made by pasture experts in the various fields being considered. The resulting material, (case studies on successful practices etc) was produced as a pasture manual.
Outcomes  This project has resulted in a high quality book of some 435 pages, which has been well accepted by the farming community.
Implications  This book provides, in easily accessible format, all the information which is needed for farmers to establish and maintain pastures in all the temperate regions of Australia. Its use should result in more profitable animal production, and the improvement of crop yields (particularly of grain crops). The information provided should allow the better establishment and management of a wide range of pasture types, and should arrest the decline in general knowledge of pastures which is currently so clearly lacking in younger farmers.

 

Completed Projects (prior to 2005-2006) - Availability of Technical and Economic Information

Project Title
Lucerne seed check: a management support system approach to improving lucerne seed profitability
RIRDC Project No: DAN-75A
Researcher:  R B Walker
Organisation: NSW Agriculture
Phone: <