Research Project Details

Project Details

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Rapid typing of Pasteurella multocida
Summary This project will build on the recently established MultiLocus Sequence Typing (MLST) scheme developed by this team (using RIRDC funding) for Pasteurella multocida. The rapid techniques (Single Nucleotide Polymorphisms – SNPs and High Resolution Melt Analysis – HRM), used by this research team for Campylobacter typing, will be developed for the rapid, cost effective first level screening of P. multocida isolates. The new rapid methods will predict MLST results and reduce the need for full sequencing (seven genes with 500 bases per gene). The results can then be used to select isolates for full MLST and/or serotyping, improving on the current situation where all isolates have to be fully serotyped or fully MLST typed (both expensive and time consuming methods). This project will deliver be a more rapid and costeffective typing scheme for P. multocida.
Program Chicken Meat
Research organisation The University of Queensland
Objective summary A) Identify SNPs that are mathematically shown to provide maximum predictive power for all seven genes in the P. multocida MLST scheme.
B) Validate SNPs by comparing results from SNP analysis with full MLST using sets of epidemiologically related and unrelated strains of P. multocida.
C) Develop HRM methodology to predict MLST results using a set of strains that are fully MLST typed.
D) Validate HRM methodology for predicting MLST results using sets of epidemiologically related and unrelated strains of P. multocida.
E) Provide ongoing typing service – including MLST and serotyping – for free to industry during the life of the project.
Project Stage Closed
Project start date Saturday, May 01, 2010
Project completion date Monday, December 19, 2011
Journal articles from project Not Available
National priority Safeguarding Australia
Rural priority Adoption of R&D
RIRDC goal CME-Increase the productivity and efficiency of chicken meat production
Principal researcher Conny Turni
Research manager Michael Beer
Admin contact Blake Chapman